Estación Experimental de Aula Dei
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Cell and Tissue Culture

 

  Objectives Papers Grants Patents

 

 

 

Dr. Luis Cistué Solá*, Begoña Echávarri Raquin

Beatriz Bueno Márquez, Patricia Fustero Abad, Diego Pescador Abad

 

* Research Group Leader

lcistue@eead.csic.es

 

General objective

 

Cereal in vitro culture. Production of doubled haploid plants. Study of QTLs in barley.

The Group mainly works with barley, bread wheat, durum wheat and rice.

The main task has been to develop a doubled haploids production technique using the cereals afore mentioned, by means of anther culture or microspore culture. The technique has been improved progressively to lead on to new culture media and technologies.

When great populations of doubled haploid have been produced from distant F1 crosses, these populations have been analyzed by molecular markers like SNPs that let us make genetic maps and localize characters of agronomic interest.

 

Specific objectives

 

The anther and microspore culture techniques work well in barley, wheat and rice. However, the findings show a very high dependency of the genotypes.

Our research tries to improve the efficiency of anther and microspore culture with the aim of obtaining higher microspore division in relation to microspores inoculated; more developed embryos from the dividing microspores; and finally a higher percentage of green vs. albino doubled haploid plants regenerated.

The three afore mentioned points analyzed have been improved by changing the growth conditions of donor plants, and the technology used in the in vitro culture, and adding several substances to the induction media.

The development of new barley varieties is the final aim of this group. In this regard we are working in the production of new feed barley varieties with high yield. This can be obtained either by obtaining lines with high yield or by obtaining resistant lines to drought stress or diseases.

We are also working with malting barleys and barley for human food. As far as barley for human food is concerned, we are trying to obtain hulless lines with high content of betaglucan fiber.

We are in a permanent and substantial collaboration with the Prof. Pat Hayes for the Oregon University (OSU). We often exchange barley vegetal material. Both laboratories produced and nowadays are maintaining the Oregon Wolfe Barley collection. It is a population of barley with mutant genes which is very useful for studying and locating molecular markers.

For the production of new varieties of bread and durum wheat, we are collaborating with the Companies: Batlle Seeds S.A. (Bell lloc, Lérida) and Agromonegros S.A. (Leciñena, Zaragoza). We are producers of lines for these companies, and at the same time they let us use their net trials for assaying our own lines.

In relation to durum wheat, our Group developed a technique for microspore culture which is able to produce green doubled haploids, while most laboratories are using the inter-generic crossing with maize due to the technical difficulties.

Besides, we are working with the Company COPSEMAR for the production of rice doubled haploids. More of 1,000 DH lines have been already produced and are being assayed.

 

Representative research papers

 

SCI papers: 

 

Cistué L, I Romagosa, F Batlle and B Echávarri (2009) “Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L..) through isolated microspore culture”. Plant Cell Reports 28:727-735

 

Muñoz-Amatriaín M, L Cistué, Y Xiong, H Bilgic, AD Budde, MR Schmitt, KP Smith, PM Hayes and GJ Muehlbauer (2010) “Structural and functional characterization of a winter malting barley”. Theoretical Applied Genetics 20:971-984

 

Cistué L, A. Cuesta-Marcos, S Chao, B Echávarri, Y Chutimanitsakun, A Corey, T Flichkina, N García-Mariño, I Romagosa and PM Hayes (2011) “Comparative mapping of the Oregon Wolfe Barley using doubled haploid lines derived from female and male gametes”. Theoretical Applied Genetics 122:1399-1410

 

Chutimanitsakun Y, RW Nipper, A. Cuesta-Marcos, L Cistué, A Corey, T Flichkina, EA Johnson and PM Hayes (2011) “Construction and application for QTL analysis of a Restriction Site Associated DNA (RAD) linkage map in barley. BMC Genomics 12(4) :1-13.

 

Farré A, I Lacasa-Benito, L Cistué, JH de Jong, I Romagosa and J Jansen. (2011) “"Linkage map construction involving a reciprocal translocation". Theoretical Applied Genetics 122:1029-1037

 

Farré A, A. Visioni, I Lacasa-Benito, L Cistué, J Jansen and I Romagosa (2012) “"Agronomic effects of a reciprocal translocation in a widely grown Spanish barley variety". Euphytica 185:119-112

 

Farré A, A Cuadrado, I Lacasa-Benito, L Cistué, I Schubert, J Comadran, J Jansen and I Romagosa.(2012) "Genetic characterization of a reciprocal translocation present in a widely grown barley variety". Molecular Breeding 30:1109-1119

 

Fisk SP, A Cuesta-Marcos, L Cistué, J Russell, KP Smith, S Baezinger, Z Bedó, A Corey, T Flilichkina, I Karsai, R Waugh and PM Hayes.(2013) "FR-H3. A new QTL assist in the development of fall-sown barley with superior low temperature tolerance". Theoretical and Applied Genetics 126:335-347

 

 

Current research grants and contracts (active)

 

"Desarrollo de materiales y técnicas para el análisis integrado de la respuesta de la cebada (Hordeum vulgare L.) al estrés abiótico". Plan Nacional de Recursos y Tecnologías Agroalimentarias. CSIC. – UdL – IRTA (Proyecto Coordinado) 2006-2008 Investigador principal: Luis Cistué Solá AGL- 20054C5251

 

"Desarrollo de materiales y métodos de interés para el estudio de estreses en cebada (Hordeum vulgare L.)". Plan Nacional de Recursos y Tecnologías Agroalimentarias. CSIC. – UdL – IRTA (Proyecto Coordinado) 2009-2011 Investigador principal: Luis Cistué Solá AGL-20084C4031

 

"Desarrollo de nueva metodología y mejora de germoplasma de cebada (Hordeum vulgare L.) para pienso y alimentación". Plan Nacional de Recursos y Tecnologías Agroalimentarias. CSIC. – UdL – IRTA- Oregon State University (Proyecto Coordinado) 2012 - 2014 Investigador principal: Luis Cistué Solá AGL - 20114C0751

 

Contrato CSIC- Agromonegros S.A. para la producción de doble-haploides de cebada, trigo panadero y trigo duro. Entidades participantes: C.S.I.C. - Agromonegros S.A. Duración: 2 años Abril 2007- Marzo 2009 (prorrogado hasta 2011) Investigador responsable: Luis Cistué Solá Número de investigadores participantes: 2 PRECIO TOTAL DEL PROYECTO: 18.000 euros

 

Contrato CSIC-Semillas Battle S:A. para la producción de doble-haploides de trigo panadero y trigo duro. Entidades participantes: C.S.I.C. - Semillas Batlle S.A. Duración: 2 años Diciembre 2007 - Noviembre 2009 (prorrogado hasta 2011) Investigador responsable: Luis Cistué Solá Número de investigadores participantes: 2 PRECIO TOTAL DEL PROYECTO: 18.000 euros

 

Título del contrato: Contrato CSIC- COPSEMAR S.A. para la producción de doble-haploides de arroz. Entidades participantes: C.S.I.C. - COPSEMAR S.A. Duración: 2 años Abril 2008 - Marzo 2010Investigador responsable: Luis Cistué Solá Número de investigadores participantes: 2 PRECIO TOTAL DEL PROYECTO: 36.000 euros

 

Título del contrato: Contrato CSIC- INIA Uruguay. para la producción de doble-haploides de trigo blando a partir de material F1 uruguayo. Entidades participantes: C.S.I.C. – Instituto Nacional de Investigación Agropecuaria (Uruguay). Duración: 1 año Febrero 2009 - Enero 2010 Investigador responsable: Luis Cistué Solá Número de investigadores participantes: 2 PRECIO TOTAL DEL PROYECTO: 10.000 euros

 

Contrato CSIC-MillerCoors (Beer Company) para la producción de doble-haploides de cebada maltera. participantes: C.S.I.C. – MILLER-COORS. Duración: 1 año Octubre 2010 - Abril 2011 Investigador responsable: Luis Cistué Solá Número de investigadores participantes: 2 PRECIO TOTAL DEL PROYECTO: 20.000 dólares

 

Contrato CSIC-Semillas Battle S:A. para la producción de doble-haploides de trigo panadero y trigo duro. Entidades participantes: C.S.I.C. - Semillas Batlle S.A.. Duración: 2 años Enero 2012 - Diciembre 2013 Investigador responsable: Luis Cistué Solá Número de investigadores participantes: 2 PRECIO TOTAL DEL PROYECTO: 25.000 euros

 

 

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