Niels Sandal, Department of Molecular Biology and Genetics, Aarhus University, Denmark.
With more than 640,000 annotated insertions, the non-transgenic Lotus retrotransposon 1 (LORE1) insertion mutant resource offers knock-out alleles of the majority of active genes in the legume Lotus japonicus (http://lotus.au.dk) (Malolepszy et al., 2016). This resource has been used for forward and reverse genetics. In forward genetics two lines with variegated leaves (white sectors) were found. We identified the mutation as a new type of variegation mutation (snowy cotyledons 2, sco2). In addition, examples of use of the LORE1 resource in reverse genetics in characterization of nodulation signalling will be presented.